Vaginitis Research Today is a free monthly online journal that collates and summarizes the latest research about Vaginitis, including details on bacterial vaginitis, candida, symptoms, treatment,.

Primary biliary cirrhosis following lactobacillus vaccination for recurrent vaginitis.

Bogdanos D, Pusl T, Rust C, Vergani D, Beuers U

Institute of Liver Studies, King's College, London School of Medicine at King's College Hospital, London, United Kingdom.

BACKGROUND/AIMS: Antimitochondrial antibodies directed against the E2 subunit of the pyruvate dehydrogenase complex, PDC-E2, and other mitochondrial 2-oxoacid dehydrogenases (AMA-M2) are the hallmark for diagnosis of primary biliary cirrhosis (PBC). AMA-M2 formation as an early step in the pathogenesis of PBC has recently been assumed to be triggered by bacterial mimics of the E2 subunit and certain reactant xenobiotics. We report a case of symptomatic PBC diagnosed after sequential immunization with a lactobacillus vaccine for recurrent vaginitis over years. METHODS: Serum AMA-M2 specificity of the patient was evaluated by indirect immunofluorescence, immunoblotting and ELISA. Serum antibody responses against pyruvate dehydrogenase complex-E2 subunit (PDC-E2(212-226)), the major PBC-specific mitochondrial autoepitope, and microbial mimics revealed cross-reactivity with beta-galactosidase of Lactobacillus delbrueckii (LACDE BGAL(266-280)) which shows a high local homology with that of Lactobacillus species administered via the vaccine. The relative affinity of antibody reactivity to LACDE BGAL(266-280) was significantly higher than that against human PDC-E2(212-226). CONCLUSIONS: We conclude that lactobacillus vaccination therapy may be another culprit for the development of PBC in genetically susceptible women.

Published 22 July 2008 in J Hepatol.
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Articles on Vaginitis published 16 July 2008:

A potential role for tissue kallikrein-related peptidases in human cervico-vaginal physiology.   Biol Chem.

Abstract Human tissue kallikrein-related peptidases (KLK) are a family of 15 genes located on chromosome 19q13.4, which encode secreted serine proteases with trypsin-like and/or chymotrypsin-like activity. Relatively large levels of many KLKs are present in human cervico-vaginal fluid (CVF) and in the supernatant of cultured human vaginal epithelial cells. Many KLKs are also hormonally regulated in vaginal epithelial cells, particularly by glucocorticoids and estrogens. The physiological role ... [Abstract] [Full-text]

Articles on Vaginitis published 14 July 2008:

Analysis of vaginal lactobacilli from healthy and infected Brazilian women.   Appl Environ Microbiol, 74(14): 4539-42.

Culture-dependent PCR-amplified rRNA gene restriction analysis and culture-independent (PCR-denaturing gradient gel electrophoresis) methodologies were used to examine vaginal lactobacilli from Brazilian women who were healthy or had been diagnosed with vulvovaginal candidiasis (VVC) or bacterial vaginosis. Only Lactobacillus crispatus was detected accordingly by both methods, and H(2)O(2)-producing lactobacilli were not associated with protection against VVC. [Abstract] [Full-text]

Articles on Vaginitis published 11 July 2008:

Transcriptional profiling of the Candida albicans Ssk1p receiver domain point mutants and their virulence.   FEMS Yeast Res.

The Ssk1p response regulator of Candida albicans is required for oxidant adaptation, survival in human neutrophils, and virulence in a disseminated murine model of candidiasis. We have previously shown that the amino acid residues D556 and D513 of the Ssk1p receiver domain are critical to the Ssk1p in oxidant stress adaptation and morphogenesis. Herein, transcriptional profiling is used to explain the oxidant sensitivity and morphogenesis defect of two point mutants (D556N and D513K, ... [Abstract] [Full-text]

Articles on Vaginitis published 7 July 2008:

Efficacy and tolerance of metronidazole and miconazole nitrate in treatment of vaginitis.   Int J Gynaecol Obstet.

OBJECTIVE: To evaluate the efficacy and tolerability of a vaginal pessary containing 750 mg of metronidazole and 200 mg of miconazole nitrate used daily for 7 days in the treatment of vaginitis. METHODS: Ninety-two women with vaginitis participated in this phase 3 study using one vaginal pessary daily for 7 days. Gynecological and microbiological evaluations were carried out prior to and following treatment. RESULTS: Reductions occurred in symptoms and signs of vaginitis. Clinical cure rate was ... [Abstract] [Full-text]

A longitudinal study of vaginal douching and bacterial vaginosis--a marginal structural modeling analysis.   Am J Epidemiol, 168(2): 188-96.

The etiology of bacterial vaginosis is unknown, and there are no long-term therapies for preventing this frequently recurring condition. Vaginal douching has been reported to be associated with bacterial vaginosis in observational studies. However, this association may be due to confounding by indication--that is, confounding by women douching in response to vaginal symptoms associated with bacterial vaginosis. The authors used marginal structural modeling to estimate the causal effect of ... [Abstract] [Full-text]

Articles on Vaginitis published 27 June 2008:

Two Cases of Vaginitis Caused by Itraconazole-Resistant Saccharomyces cerevisiae and a Review of Recently Published Studies.   Mycopathologia, 166(1): 47-50.

Genitourinary infections caused by non-Candida yeasts are uncommon, and especially due to Saccharomyces cerevisiae. We describe the cases of two adult females with vulvovaginal infections caused by itraconazole-resistant S. cerevisiae who made a full recovery after oral fluconazole therapy. We also provide a concise review of recently published studies on this topic. [Abstract] [Full-text]

Articles on Vaginitis published 23 June 2008:

Current trends in hormone replacement therapy: perceptions and usage.   Climacteric, 11(3): 192-200.

OBJECTIVE: To determine whether physicians' confidence in the use of hormone replacement therapy (HRT) for climacteric symptoms has been affected by the negative media interpretation of data from landmark studies investigating HRT usage such as the Women's Health Initiative (WHI) study. METHODS: A structured questionnaire was completed via the internet by European and US gynecologists, obstetrician/gynecologists and general practitioners - all experienced in treating women with climacteric ... [Abstract] [Full-text]

Articles on Vaginitis published 19 June 2008:

Local Th1/Th2 cytokine expression in experimental murine vaginal candidiasis.   J Huazhong Univ Sci Technolog Med Sci, 28(3): 352-5.

In order to investigate the expression of Th1 and Th2 cytokines in the vaginal candidiasis caused by Candida, the fungal vaginitis model was established in female ICR mice by intravaginal inoculation of suspension of C. albicans after the animals were pretreated with estradiol. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of IL-2, IL-4, IL-10 and TGF-beta1 in the vagina in the mice of different groups at different time points after the beginning of ... [Abstract] [Full-text]

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